This workshop provides a solid basis for the understanding of the growth of animal cells and combines lecture and laboratory exercises. Topics include: Introduction to cell and tissue culture, serum containing media and the use of serum cell counting and viability determination, development of growth curves, growth of adherent and suspension cells, transfection technologies for recombinant protein expression in animal cells, cryopreservation and cryothawing    of animal cells, cell cloning and primary cell culture. The workshop also contains sections on serum-free media, attachment, adaptation/selection of adherent /suspension culture. The workshop is designed to provide a solid understanding of cell culture through classroom presentation and laboratory work.